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Feyond-A300/A400/A500 Microplate Reader

Wavelength accuracy: Absorbance: 200-1000 nm
Measure range: 0-4 OD
Screen size: 10 inch
Model:
Feyond-A300
Feyond-A400
Feyond-A500
Availability:
Inquire

  • Feyond-A300/A400/A500

  • NADE

  • 1

  • CHINA

Product Description

Function Configuration Overview


Monochromater Type
Model Feyond-A300 Feyond-A400 Feyond-A500
Absorbance-monochromater ● ● ●
Absorbance-filter


Fluorescence-filter ● ● ●
Time-resolved fluorescence
● ●
Fluorescence
polarization-filter


●
Luminescence ● ● ●
u-Nano Plate ● ● ●
Injector (optional) ○ ○ ○


Feyond-A

Feyond-A application


Features

● UV/Vis Absorbance

Wavelength selection is done by using an advanced monochromator system. Any wavelength between 200 to 1000 nm can be selected. Using the spectral scanning feature, the whole spectrum of a sample be scanned in 1 nm increments to allow identification of the optimal measurement wavelength for a new assay.

● Fluorescence

Instrument is equipped with filter-based fluorescence optics and dichroic mirrors for screening applications such as fluorescence polarization, and TR-FRET. Standard applications such as fluorescence-based DNA/RNA quantification assays are not only supported in microplates, but also in low volume u-Nano ultra-micro plate.
The independent removable filter modules make it more convenient for users to replace the filter. The filter-based fluorescence optics detection ensures high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent blockage of undesired wavelengths. This is ideal for excitation and emission applications. The filters are also the technically preferred and most cost efficient technology for non-abosrbance based assays.

● Luminescence

Feyond series luminescence microplate readers show excellent sensitivity and wide dynamic range in both glow and flash based assays. The PMT enhances the maximum sensitivity of weak luminescence signals, prevents oversaturation of high signals, effectively improves the detection range of luminescence. The optimized light path minimizes signal crosstalk between holes and ensures the accuracy of experimental results. The precise dual-channel injector ensures assay performance even when assaying high-density 384-well plates.

● Time-Resolved Fluorescence (TRF)

Time-resolved fluorescence is based on lanthanide elements as dyes. When excited, the emission time is much longer than that of ordinary fluorescein. After the excitation light is turned off, the emitted light can still be continuously expressed and released, thereby eliminating the interference of excitation light and scattered light.
Time-resolved fluorescence has high sensitivity, strong specificity, good stability, and short operation flow. It is suitable for ultra-micro analysis in biology and medicine, hormone detection, viral hepatitis marker detection, target cell marker detection, and drug screening.

● Fluorescence Polarization (FP)

The optimized optical path design of Feyond-A500 combined with the performance of the fast switching polarizer can effectively reduce the detection deviation. The 10-inch lower computer touch screen can improve the flexibility of experimental parameter setting. This function is often used to detect the interaction between small molecules and macromolecules, such as the determination of drugs and hormones, tyrosine kinase detection, receptor/ligand research, protein/polypeptide interactions, DNA/protein interactions, etc.


Specifications

Multi-Mode Microplate Reader Selection Guide
ModelFeyond-A300Feyond-A400Feyond-A500
Plate6-384
Absorbance✓✓✓
Fluorescence✓✓✓
Luminescence✓✓✓
Time-resolved fluorescence--✓✓
Fluorescence polarization----✓
Xenon lamp✓✓✓
Wavelength rangeAbsorbance: 200-1000 nm
Fluorescence: EX: 200-1000 nm; EM: 270-850 nm
Luminescence: 200-850 nm
----Fluorescence polarization: 300 - 850 nm
Wavelength selectionAbsorbance: monochromator / fluorescence: filter
Incubation temperatureRT+4 °C~45 °C
Screen size10 inch
SystemAndroid system
Analysis softwareReader It-II
AccessoriesMicroplate, injector


Technical Parameter

Model

Feyond-A300

Feyond-A400

Feyond-A500

Absorbance

Light source

Xenon lamp

Detector

PD

Wavelength

accuracy

2 nm

Wavelength

repeatability (SD)

200-1000 nm, 1 nm step

Half width (FWHM)

<2.5 nm

Wavelength range

200-1000 nm, 1 nm step

Measure range

0-4 OD

Resolution

0.0001 OD

Accuracy @450 nm

96-precision mode: ± (1.0 %+0.003 Abs) @(0.0-2.0 Abs]

±2.0 % @(2.0-3.0 Abs]

Repeatability @450 nm

CV < 1.0 % or SD < 0.003 fast (0.0-3.0Abs]

CV < 0.5 % or SD < 0.003 accurate (0.0-3.0Abs]

Stray light

0.1% @220 nm

Linear @450 nm

R2 ≥ 0.999 @[0.0 - 3.0 Abs]

Reading time

96-well plate: fast <15 s

Luminescence


Detector

PMT

Detection limit

15 amol/hole; 5 amol/hole (photon PMT)

Linear dynamic

range

6 logs

Crosstalk

≤ 0.005 %

Wavelength range

200-850 nm

Fluorescence


Reading mode

Top reading

Excitation light

source

Xenon lamp

Detector

PMT

Wavelength range

EX: 200-1000 nm; EM: 270-850 nm

Filter EX/EM

3 groups: EX470/EM525, EX523/EM564, EX624/EM692 (other wavelengths can be replaced)

Detection limit

1 pM

Linear dynamic

range

6 logs

TRF


Wavelength range

--

EX: 200-1000 nm; EM: 270-850 nm

Detection limit

--

0.05 pM

FP

Wavelength range

--

--

λ1-λ2

Detection limit

--

--

5 mP


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